FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006184351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006184351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062399
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTGG33350.16170488833635005No Hit
ATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTC32300.15661372993295672No Hit
CTATAAGAGGTTGTCATTGCAGCATTCCTGGATACAACATTGCTAGTCTT31360.15205593098134745No Hit
CTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTT28730.1393037913614194No Hit
AGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGG26880.13033365512686923No Hit
CAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTTTTG26670.12931542344619057No Hit
AAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAG26460.1282971917655119No Hit
ATAAGAGGTTGTCATTGCAGCATTCCTGGATACAACATTGCTAGTCTTAC25910.1256303945065916No Hit
ATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTCTCACCTT25360.12296359724767128No Hit
AGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAA24980.12112108277787179No Hit
AATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGT24900.1207331849947561No Hit
AAGAGGTTGTCATTGCAGCATTCCTGGATACAACATTGCTAGTCTTACCT24820.12034528721164044No Hit
CCCACATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACA23940.11607841159736793No Hit
AAAAGGTGAGACTTTAACCCTATAAGAGGTTGTCATTGCAGCATTCCTGG23600.11442984601912627No Hit
CACATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAAC23520.11404194823601058No Hit
CAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAG23480.11384799934445275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23380.11336312711555814No Hit
AGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTT22620.10967809817595917No Hit
ATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGTTAAAGTC22130.1073022242543756No Hit
CTTTAACCCTATAAGAGGTTGTCATTGCAGCATTCCTGGATACAACATTG22040.10686583924837047No Hit
ACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACCTCTTATAGGGT21860.10599306923636018No Hit
CTATACTTCTTTTGCCAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTT21690.10516878644723936No Hit
AAGGTGAGACTTTAACCCTATAAGAGGTTGTCATTGCAGCATTCCTGGAT21530.10439299088100801No Hit
AAGATAAAGTCCTCTTATCATTTTGCCTTTGTGTCCTATAGTGGAACACC21430.10390811865211338No Hit
CTATGTGGGCTACCCCACATAGGTAAGACTAGCAATGTTGTATCCAGGAA21390.10371416976055554No Hit
ACATAGGTAAGACTAGCAATGTTGTATCCAGGAATGCTGCAATGACAACC21170.10264745085698743No Hit
CCTATAAGAGGTTGTCATTGCAGCATTCCTGGATACAACATTGCTAGTCT21170.10264745085698743No Hit
CTATACTTCTTTTGGCAAAAGAAGTATAGCCAACAAGATAAAGTCCTCTT21040.10201711695942443No Hit
CTATGTGGGGTAGCCCACATAGGTAAGACTAGCAATGTTGTATCCAGGAA21020.1019201425136455No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)