FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006184362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006184362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436923
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGCGGCTCCGCCCCGCGCGCACTATTTAATCCCAGAATGCCTCTGTG43270.30112956644162564No Hit
CACTATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCG37170.25867774404056443No Hit
CTATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTT36560.2544325618004583No Hit
AGAAGTGCGCAGGCGCGGCTCCGCCCCGCGCGCACTATTTAATCCCAGAA34700.24148823562570856No Hit
AGTCTCCATGCTCTCCATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCG30280.21072806267280847No Hit
CTGCATCTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTCGCC26470.18421307196001455No Hit
ACTATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGT25630.178367247235934No Hit
ATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCG25290.17600108008571091No Hit
GCACTATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACC24530.17071200057344757No Hit
ATCTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTCGCCATCT23630.1644486169405041No Hit
TATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTC23210.16152570457846382No Hit
CTGCGCACTTCTGCCGTGCTTACGCCCTCTAGCGAGGCAGAAGTGCGCAG22230.1547055757337032No Hit
GGCTCCGCCCCGCGCGCACTATTTAATCCCAGAATGCCTCTGTGTCGCAT22000.15310493324972876No Hit
CCCCGCGCGCACTATTTAATCCCAGAATGCCTCTGTGTCGCATCAGACTT21420.1490685304640541No Hit
CTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGCTGAGGGGGCCGG20980.1460064317990595No Hit
CTGCGCACTTCTGCCTCGCTAGAGGGCGTAAGCACGGCAGAAGTGCGCAG20900.14544968658724233No Hit
CTCCGCCCCGCGCGCACTATTTAATCCCAGAATGCCTCTGTGTCGCATCA20650.1437098578003136No Hit
ATCTTGACGCCGCCGTACCGGCCCCCTCAGCTTCCGAACGGTGCGGCAAG20550.1430139262855421No Hit
CATCTTGACGCCGCCGTACCGGCCCCCTCAGCTTCCGAACGGTGCGGCAA20530.1428747399825878No Hit
AAGATGGCGACGGCAGTCTCCATGCTCTCCATCTCCAGCACTTGAAAAGA20120.14002142077202467No Hit
GGCTGCATCTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTCG19920.13862955774248167No Hit
CAGAAGTGCGCAGGCGCGGCTCCGCCCCGCGCGCACTATTTAATCCCAGA19820.1379336262277102No Hit
TAGAGGGCGTAAGCACGGCAGAAGTGCGCAGGCGCGGCTCCGCCCCGCGC19750.13744647416737013No Hit
AGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGCTGAGG19640.1366809495011215No Hit
CATCTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTCGCCATC19390.13494112071419276No Hit
AAGATGGCGGCGGCTGTGGCGGCGGCAGCTGCCGCTGCGGCCGCGGCTGC19340.13459315495680702No Hit
ATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTGCCGC18800.13083512477704093No Hit
AATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAG18180.12652034938545767No Hit
CTAGAGGGCGTAAGCACGGCAGAAGTGCGCAGGCGCGGCTCCGCCCCGCG18160.12638116308250338No Hit
AGCTGCCGCTGCGGCCGCGGCTGCATCTTTTCAAGTGCTGGAGATGGAGA18020.12540685896182327No Hit
CAGGCGCGGCTCCGCCCCGCGCGCACTATTTAATCCCAGAATGCCTCTGT17680.12304069181160021No Hit
CTCCATGCTCTCCATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAG17580.12234476029682873No Hit
ATGGAGACTGCCGTCGCCATCTTGACGCCGCCGTACCGGCCCCCTCAGCT17200.11970022054069702No Hit
AATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGCTGAGGGG17060.11872591642001695No Hit
CATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTGCCG16940.11789079860229114No Hit
CAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGCTGAG16810.1169860876330882No Hit
ATCAGACTTGCCGCACCGTTCGGAAGCTGAGGGGGCCGGTACGGCGGCGT16740.11649893557274815No Hit
AGCCGCGGCCGCAGCGGCAGCTGCCGCCGCCACAGCCGCCGCCATCTTGA16680.11608137666388525No Hit
AGAGGGCGTAAGCACGGCAGAAGTGCGCAGGCGCGGCTCCGCCCCGCGCG16650.11587259720945381No Hit
CTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTGCCGCCG16470.11461992048286514No Hit
CAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTGCCGCCGCCA16280.11329765060479928No Hit
CTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTGCCGCCGCCACAGCC16220.11288009169593638No Hit
ATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGC15960.1110706697575305No Hit
TAATCCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAA15800.10995717933389611No Hit
TGCATCTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTCGCCA15750.10960921357651036No Hit
AAGCACGGCAGAAGTGCGCAGGCGCGGCTCCGCCCCGCGCGCACTATTTA15650.10891328206173888No Hit
CATGCTCTCCATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGG15380.10703426697185583No Hit
GTCAAGATGGCGGCGGCTGTGGCGGCGGCAGCTGCCGCTGCGGCCGCGGC15260.10619914915413005No Hit
CTGGAGATGGAGAGCATGGAGACTGCCGTCGCCATCTTGACGCCGCCGTA15070.10487687927606419No Hit
CTCCATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTG15000.10438972721572416No Hit
CCCAGAATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGCTG14930.10390257515538412No Hit
AGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGCAGCTGCCGCCGCCAC14750.10264989842879542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14610.10167559430811533No Hit
ATGCCTCTGTGTCGCATCAGACTTGCCGCACCGTTCGGAAGCTGAGGGGG14510.10097966279334385No Hit
ATGCTCTCCATCTCCAGCACTTGAAAAGATGCAGCCGCGGCCGCAGCGGC14490.10084047649038953No Hit
AGGGCGTAAGCACGGCAGAAGTGCGCAGGCGCGGCTCCGCCCCGCGCGCA14480.10077088333891239No Hit
CTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTCGCCATCTTG14420.10035332443004948No Hit
GGCAGTCTCCATGCTCTCCATCTCCAGCACTTGAAAAGATGCAGCCGCGG14410.10028373127857235No Hit
CGGCTGCATCTTTTCAAGTGCTGGAGATGGAGAGCATGGAGACTGCCGTC14400.10021413812709519No Hit
GTAAGCACGGCAGAAGTGCGCAGGCGCGGCTCCGCCCCGCGCGCACTATT14370.10000535867266375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)