FastQCFastQC Report
Fri 31 May 2024
EGAF00006196767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006196767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12858747
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGCGAGTGTAGATCT608080.47289210993886105Illumina Single End Sequencing Primer (100% over 32bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG370140.2878507524877813No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA214700.16696805684099703No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA178850.13908820198422134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)