FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006201261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006201261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103344972
Sequences flagged as poor quality0
Sequence length20-61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGTA3577070.3461290792163551Illumina RNA PCR Primer (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1383320.13385460107338362No Hit
GGGTAACGAAGCGAACGTGGCGAACTGAAACATCTAAGTAGCTACAGGAAAAGAAATCAA1379480.13348303002104447No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTA1354940.1311084587646896No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGA1159980.11224348679488733No Hit
GCCGTAAGGTCATCGTTATCTGAATACATAGGGTAACGAAGCGAACGTGGCGAACTGAAA1146430.11093234414926349No Hit
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTGGCAAACGAGCTTTGATCCGCAGATTTC1125610.10891773234986217No Hit
GTGTGGAACTAGGCTTGCGACAAGTAGGGCGGGACACGTGAAATCCTGTCTGAACATGGGG1092140.10567906487022899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)