FastQCFastQC Report
Sat 9 Jul 2022
EGAF00006204854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006204854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413595273
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG259746066.280198951886957Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT4205360.10167814466293476Illumina Single End PCR Primer 1 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)