FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006204896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006204896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31693014
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGGGTGGAATCAATTTTTTTAATTTTCCTACTAAATTTTAC1087250.34305667488740577No Hit
GTTCTGGATTCAGTAAAATTTAGTAGGAAAATTAAAAAAATTGATTCCAC919110.2900039737463909No Hit
GTCTGAATGTCCGTACTTCTCCAAAATTTCTTTCTCATGGCAAAGTTCTT797900.2517589523041261No Hit
GTCTTCAGGGTGTTTAGAAAACCAGCTACCTGCCTTTCTGGAAGAACTTT766450.2418356297700181No Hit
ATGCTGGAGTGGGCTTTTTGTGTGCAAGAAAACAGTTATGTCTTCCCTCT604530.1907455062494214No Hit
TCTTTGGGCTGCTCGCTATGACAAAATAATTCCATCTTGCTGCAAAGCTG554770.17504488528607598No Hit
AAATAATTCCATCTTGCTGCAAAGCTGAAAATGCAGTTGAATGCTTCCAA515880.16277404225423306No Hit
GTTTGGAAGCATTCAACTGCATTTTCAGCTTTGCAGCAAGATGGAATTAT500580.1579464799403427No Hit
CTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGT461720.14568510271695836No Hit
GCTTACTTCCTTGTAAGTGGCTTCTTGAACAAACTGGGCAAAAAATATGG411030.1296910416913961No Hit
TTTCTATGCAGTGTTCTGGATTCAGTAAAATTTAGTAGGAAAATTAAAAA410830.12962793630167205No Hit
CCCTGAAGACTGTTCATCTCCAGTGGGTTTCTCAATTGCAGTCAATGCAT393990.1243144624869064No Hit
GGACTAGTTTCTGGATTTCAGTAAAATTAACTTTGGTAAACTTCTGACTC392830.12394845122650688No Hit
TGTCCGTACTTCTCCAAAATTTCTTTCTCATGGCAAAGTTCTTCCAGAAA391890.12365185589480383No Hit
CAAAAAGCCCACTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTG374400.11813328956343501No Hit
CAGGAAGGGATGCCTTCTTGCTATCTCATAAATGAATTTGTTCATGAATG365150.11521466528869738No Hit
TGCATGTGCAGTAATGAAAAATTTTGGGACCCGAACTTTCCAAGCCATAA347890.10976867015551124No Hit
ATCCAGGACTAGTTTCTGGATTTCAGTAAAATTAACTTTGGTAAACTTCT321830.1015460378744666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)