FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006204916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006204916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14731021
Sequences flagged as poor quality0
Sequence length95
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT267360.1814945481375663No Hit
ATGCTGGAGTGGGCTTTTTGTGTGCAAGAAAACAGTTATGTCTTCCCTCT247960.16832506042860165No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA243870.16554860657655707No Hit
CTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGT223620.1518021052308594No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA220460.14965697218135798No Hit
GTCTTCAGGGTGTTTAGAAAACCAGCTACCTGCCTTTCTGGAAGAACTTT209960.142529156668774No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA207430.1408116925500276No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT206010.13984774035689718No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG204130.13857152196035835No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC201410.1367250783228128No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC197170.13384679853487413No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG191900.13026931398712963No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA188110.12769651200687313No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA186970.1269226348940783No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT186360.12650854275477577No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA183530.12458742676424124No Hit
CCCTGAAGACTGTTCATCTCCAGTGGGTTTCTCAATTGCAGTCAATGCAT183220.12437698649672686No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC181290.12306682612155669No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA178810.12138330398144162No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT178570.12122038248401111No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC173960.11809093205420046No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC173870.118029836492664No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA172920.11738493889866831No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC170920.11602725975341424No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG169810.11527374782779821No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT164100.1113975738680978No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG163960.11130253632793002No Hit
ATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCCATATTGGATT163810.11120071039203597No Hit
TCTTTGGGCTGCTCGCTATGACAAAATAATTCCATCTTGCTGCAAAGCTG160500.10895375140664044No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT156480.10622481632467974No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC150330.10204995295302341No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT150290.10202279937011835No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT148660.10091629086673626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)