FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006204939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006204939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31962939
Sequences flagged as poor quality0
Sequence length90
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGGGTGGAATCAATTTTTTTAATTTTCCTACTAAATTTTAC1081720.3384294541875514No Hit
GTCTTCAGGGTGTTTAGAAAACCAGCTACCTGCCTTTCTGGAAGAACTTT953650.2983611738582613No Hit
GTTCTGGATTCAGTAAAATTTAGTAGGAAAATTAAAAAAATTGATTCCAC924030.2890941912444284No Hit
GTCTGAATGTCCGTACTTCTCCAAAATTTCTTTCTCATGGCAAAGTTCTT918720.2874328922005577No Hit
TCTTTGGGCTGCTCGCTATGACAAAATAATTCCATCTTGCTGCAAAGCTG708890.22178498666846624No Hit
ATGCTGGAGTGGGCTTTTTGTGTGCAAGAAAACAGTTATGTCTTCCCTCT686580.21480502778546115No Hit
GTTTGGAAGCATTCAACTGCATTTTCAGCTTTGCAGCAAGATGGAATTAT587180.18370651084369932No Hit
CTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGT579160.18119735484900182No Hit
AAATAATTCCATCTTGCTGCAAAGCTGAAAATGCAGTTGAATGCTTCCAA576920.18049654319961003No Hit
CCCTGAAGACTGTTCATCTCCAGTGGGTTTCTCAATTGCAGTCAATGCAT483580.15129397205932785No Hit
TTTCTATGCAGTGTTCTGGATTCAGTAAAATTTAGTAGGAAAATTAAAAA473340.14809026166210812No Hit
TGTCCGTACTTCTCCAAAATTTCTTTCTCATGGCAAAGTTCTTCCAGAAA464740.1453996455081931No Hit
GCTTACTTCCTTGTAAGTGGCTTCTTGAACAAACTGGGCAAAAAATATGG437750.13695549085770867No Hit
CAGGAAGGGATGCCTTCTTGCTATCTCATAAATGAATTTGTTCATGAATG435960.13639546726288218No Hit
CAAAAAGCCCACTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTG410770.12851446483065904No Hit
ATCCAGGACTAGTTTCTGGATTTCAGTAAAATTAACTTTGGTAAACTTCT395950.12387784490030783No Hit
TGCATGTGCAGTAATGAAAAATTTTGGGACCCGAACTTTCCAAGCCATAA364590.1140664818088224No Hit
CTTCCAAACAAAGGCAGCAACAGTTACAAAAGAATTAAGAGAAAGCAGCT359050.11233322442595157No Hit
CTCTTAATTCTTTTGTAACTGTTGCTGCCTTTGTTTGGAAGCATTCAACT348920.10916392888651447No Hit
GGACTAGTTTCTGGATTTCAGTAAAATTAACTTTGGTAAACTTCTGACTC344790.10787180740794833No Hit
CAGTTATGTCTTCCCTCTTCACTTTGGCTGCAGCAGTCTGAATGTCCGTA337770.10567551375672932No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA324630.10156450256342198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)