FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20904996
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGCTT610256529.19189747752164No Hit
GATTCTGG537092225.69204988128197No Hit
ATGAAGAC485213423.210403867094737No Hit
CCACTACA376584118.014071851532524No Hit
ATGAAGCC1124190.5377614040203595No Hit
CCCCTACA543650.2600574522951356No Hit
GATTCTGA393260.18811771119209975RNA PCR Primer, Index 40 (100% over 8bp)
TGCGGCTA349440.16715621471537234No Hit
GCTTCTGG229060.10957189372339511No Hit
ATGAAGAA225790.10800767433775162No Hit
GATTATGG225770.10799810724670793No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)