FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21176208
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGCTT611679128.8852045654255No Hit
GATTCTGG543010425.64247574447701No Hit
ATGAAGAC487133223.00379746931084No Hit
CCACTACA379434217.91794829366995No Hit
ATGAAGCC1420150.6706347047592278No Hit
CCCCTACA709770.3351733228158696No Hit
GATTCTGA391140.1847072903704006RNA PCR Primer, Index 40 (100% over 8bp)
TGCGGCTA363180.17150379331370375No Hit
GCTTCTGG314310.14842600714915533No Hit
TGCGGCGT265290.12527738677292932No Hit
GATTATGG259490.12253846392139707No Hit
ATGAAGAA258830.12222679339001581No Hit
CCACTACC239990.11333001640331453No Hit
CCACTAAA213200.10067902619770262No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)