FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17910595
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCATCG553701130.91472393854029No Hit
ATTGGCGT481440326.880195772390586Illumina PCR Primer Index 6 (100% over 8bp)
GCAACAAA363736620.308459880869396No Hit
TAGTTGTC352053619.656164409948413No Hit
CCAACAAA657160.366911317016548No Hit
GAAACAAA258030.14406556566099563No Hit
ACAACAAA237890.13282082476880305No Hit
CGCCCTCG195420.1091086030363592No Hit
GCAAAAAA191590.10697020394911505No Hit
CGCCATCA182650.10197874498306728No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)