FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17910595
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3396741.8964975758761784Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT815240.4551719247741351Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG556400.31065411283098077No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT333300.18609097017714935Clontech SMART CDS Primer II A (100% over 25bp)
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA257510.1437752347144246No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA237330.13250816067249582No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA207530.11586996411900331No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT200270.11181649744187727No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196240.10956643260595196Clontech SMART CDS Primer II A (100% over 21bp)
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC190710.10647887465491794No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC189790.10596521221098462No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT184040.10275482193640134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)