FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16934402
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCATCG519904030.701054575177793No Hit
ATTGGCGT453325126.769477894761206Illumina PCR Primer Index 6 (100% over 8bp)
GCAACAAA342487420.224357494288846No Hit
TAGTTGTC331073019.550321292715267No Hit
CCAACAAA625710.3694904608972906No Hit
GAAACAAA298170.17607353362699196No Hit
ACAACAAA255270.15074048673227433No Hit
CGCCCTCG209820.12390162935780077No Hit
GCAAAAAA195840.11564624484525642No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)