FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16255048
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCATCG497647030.614920361970015No Hit
ATTGGCGT436215626.835700515925883Illumina PCR Primer Index 6 (100% over 8bp)
GCAACAAA329241420.254717180779778No Hit
TAGTTGTC318189619.574817619732652No Hit
CCAACAAA608080.37408686827624255No Hit
GAAACAAA314120.19324458469762748No Hit
ACAACAAA260050.1599810717261493No Hit
CGCCCTCG228080.14031333527898532No Hit
GCAAAAAA215180.13237733902723633No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)