FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782997
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1092251.4033797006474498Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333290.4282283547070621No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239980.3083388057325475Clontech SMART CDS Primer II A (100% over 24bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC86000.11049728016084293No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG85110.10935376179638769No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83280.10700248246273253Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)