FastQCFastQC Report
Sun 2 Apr 2023
EGAF00006205473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20584379
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTATG1291540.6274369510977232TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTAT496130.24102257347671263TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)