FastQCFastQC Report
Sat 1 Apr 2023
EGAF00006205492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12652800
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGCCGGTGTGTAGATCTC593280.46889226100151743Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGCCGGTGTGTAGATCT243740.1926372028325746Illumina Single End PCR Primer 1 (97% over 34bp)
GCCAGCTGAAGGAAAGAGGAAGTGCTAGAGAGAGCCCCCTTCAGTGTGCT147210.11634578907435508No Hit
CCAGTCAAGCAGAAGACGGCATACGAGATAATTCTGCGTGACTGGAGTTC133450.10547072584724329Illumina PCR Primer Index 6 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)