FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19608440
Sequences flagged as poor quality0
Sequence length91
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAA684880.34927816797256694Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA494630.2522536213997646Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT410680.20944042463347415Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG258100.13162699327432473No Hit
ATAAAATTCTTCTTAGTAGCTATTACCTTCTTATTATTTGATCTAGAAAT204640.1043632231834863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)