FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21736240
Sequences flagged as poor quality0
Sequence length91
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1167520.5371306168868213Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA702030.32297674298774764No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA524460.24128368107823617No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT449760.2069171116991715No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT447050.20567034592919473No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC405610.18660541105545392No Hit
TTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACT352990.16239699230409674No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA352400.1621255562139542Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACT322320.14828691622838172No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA321880.14808448931369914No Hit
CACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTA308260.14181845618193395No Hit
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA304820.140235845758052No Hit
CTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCT294170.13533619430039417No Hit
GCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTT283370.13036753366727638No Hit
CGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAA272700.12545868098622392No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG264220.121557362266887No Hit
CAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATC262120.12059123381044742No Hit
CAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA237900.10944855227951109No Hit
GCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGC223960.1030352995734313No Hit
ACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATC218820.100670585161003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)