FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10756394
Sequences flagged as poor quality0
Sequence length91
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAA488040.4537208287461392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGGACTCAACATA179290.16668225429451541No Hit
ATCAAATATCACTCTCCTACTTACAGGACTCAACATACTAGTCACAGCCC178510.16595710421169027No Hit
CAAATATCACTCTCCTACTTACAGGACTCAACATACTAGTCACAGCCCTA163710.15219784622987964No Hit
CAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAACAC162720.15127746343244772No Hit
CTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGGACT149620.1390986607593586No Hit
GGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTC140360.13048982772479328No Hit
CTCCTACTTACAGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTA136340.12675251575946364No Hit
CTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTA129760.1206352240351181No Hit
CAGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACC128980.11991007395229293No Hit
CTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAACACAATGGGG126300.11741853264207316No Hit
CACAGCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCAC125020.11622854276256522No Hit
ACTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAA111060.10325021563918169No Hit
GTTCTCCTGATCAAATATCACTCTCCTACTTACAGGACTCAACATACTAG109990.10225545847428051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)