FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13196542
Sequences flagged as poor quality0
Sequence length91
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT537250.40711422734834624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA350150.26533466115593013No Hit
GCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTT188850.1431056711674922No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA174090.1319209229205651Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA162090.12282763166290078No Hit
GCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGC150150.11377980686152478No Hit
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA146440.11096846431436357No Hit
CTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCC141900.10752816912188057No Hit
CTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA137890.10448949429327774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)