FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006205761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16973207
Sequences flagged as poor quality0
Sequence length91
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1102550.6495826039239373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT662460.3902974847357956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA482260.28413015878496034No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT401770.23670836041768656No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT389860.22969141895223455No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA367670.2166178730984663No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC363900.21439672538018303No Hit
TTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACT327880.193175043467036No Hit
CCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACT281790.16602048157428353No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA280230.165101385966718No Hit
CACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTA271370.1598813942468268No Hit
GCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTT259490.1528821276969049No Hit
CTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCT255380.1504606642692804No Hit
CGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAA244740.1441919608946029No Hit
CAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATC227190.13385213530949103No Hit
CAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA213440.12575113235819255No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA205250.12092588041847366Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGC199380.11746748861308297No Hit
ACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATC194410.11453934427359544No Hit
CTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCC191630.11290146876780564No Hit
CTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA191180.11263634503485406No Hit
GTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAAT191110.1125951035652838No Hit
CCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAG175030.1031213488411471No Hit
TTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTT171010.10075291016011292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)