FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18751684
Sequences flagged as poor quality0
Sequence length91
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1113460.5937920028942467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT671020.3578451940636372Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC621600.33149022775767767No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT586090.3125532618830394No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA482360.2572355634832584No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC477030.2543931521030324No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT399660.2131328578275956No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT391600.20883457720383938No Hit
GTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAG391320.208685257281426No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA369630.1971182961487619No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC364730.19450519750652795No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG355030.18933232876577913No Hit
ATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCA346390.1847247425884523No Hit
TTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTT338710.18062911043082852No Hit
CAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGC336040.1792052383135296No Hit
TTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACT332100.17710409369099864No Hit
CTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAG312250.16651837776276518No Hit
GCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATT309890.16525982413099538No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT306940.16368663209128312No Hit
CCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACG297050.15841243911746808No Hit
CCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACT284580.15176236971570128No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA284340.15163438121077552No Hit
CCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGAC280190.14942124664643452No Hit
AACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCT277360.14791204885918513No Hit
CACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTA276240.14731476916953165No Hit
TCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACC274940.14662149810118388No Hit
GACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACT262600.14004075580625186No Hit
CTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCT260310.13881953215508536No Hit
GCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTT259770.13853155801900247No Hit
CGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGAC248160.13234011409321958No Hit
CGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAA245370.1308522477234578No Hit
CTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCA243700.1299616610433495No Hit
CCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGA238540.12720990818744599No Hit
CCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTC232340.12390353847686426No Hit
CAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATC229440.12235701070901152No Hit
CTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCC229000.12212236511664766No Hit
CAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTA224100.11950926647441372No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA223450.11916263094023981No Hit
CAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA215390.11486435031648357No Hit
AGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACA215230.11477902464653308No Hit
GGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCA209960.11196861039253861No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA206790.11027809555664442Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGG203910.10874223349753548No Hit
GCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGC203020.10826760945843585No Hit
TTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCAC201060.10722237000154225No Hit
CTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCT195670.10434796149508493No Hit
ACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATC195550.10428396724262204No Hit
CTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCC193860.10338271485376993No Hit
GTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAAT193770.10333471916442279No Hit
CTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA190810.1017561942703386No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT188470.1005083063473126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)