FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17305035
Sequences flagged as poor quality0
Sequence length91
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1085440.6272394132690284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT675350.39026214046952235Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA481210.2780751382473367No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT400350.23134885309391168No Hit
GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCT387840.22411974318457029No Hit
CATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCA361810.2090778782013443No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC360370.20824575044199567No Hit
TTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACT321760.1859343248944599No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA283220.1636633500018925No Hit
CCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACT280820.1622764704029781No Hit
CACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTA272160.15727214651689522No Hit
AACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCT270130.15609907752281343No Hit
GCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTT260040.15026840454237742No Hit
CTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCT258830.14956918607792472No Hit
CGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAA245300.14175065233904466No Hit
CAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATC226070.1306382795527429No Hit
CAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGC219040.12657587806092274No Hit
CAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA212060.12254236989407996No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA208670.12058340246061333Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGC201690.11654989429377056No Hit
CTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCC194870.11260884476685543No Hit
ACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATC192750.11138376778781435No Hit
CTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA190600.11014135481378685No Hit
GTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAAT190100.10985242156401301No Hit
CCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAG175830.10160626661546769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)