FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16635781
Sequences flagged as poor quality0
Sequence length91
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT661270.3974986205937671Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA473720.28475969958969766No Hit
CACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCAT394240.23698316297864225No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA281400.1691534650522269No Hit
GCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTT258410.1553338553807603No Hit
CTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCT254730.15312175605100836No Hit
CGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAA243380.1462991127377789No Hit
CAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA211280.12700335499727966No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA202000.12142501755703565Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGC198690.11943533038815551No Hit
CTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCC190890.11474664159139868No Hit
ACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATC190290.11438597322241739No Hit
GTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAAT189090.11366463648445481No Hit
CTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA187380.11263673163285813No Hit
CCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAG173220.10412495812489957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)