FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31849721
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA2314620.7267316407575438Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1728080.5425730416916368Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1173860.36856209823627656No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC1007420.3163041836379038No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA696080.21855136501823672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAA692520.21743361582351067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA603560.18950244493507495No Hit
CCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGC456140.14321632519167124No Hit
GCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATT437590.13739209834836544No Hit
CACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCC437340.1373136047251403No Hit
GGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAG425010.13344229922767611No Hit
TTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTA414780.13023034016530316No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA407720.12801368024542506No Hit
CCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAG401110.12593830884735224No Hit
CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC390970.12275460748934032No Hit
CCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTT389770.12237783809785964No Hit
ATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCT384400.12069179507098352No Hit
AAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACC369320.11595705971804275No Hit
ATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACG357020.1120951734553656No Hit
CCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTA342540.10754882279816516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)