FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32037112
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT1158310.36155256441342154No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC992320.30974077813256073No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA689900.2153440047904443No Hit
CGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA602690.18812244998862568No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA512350.1599239032532021Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGC451950.1410707681766072No Hit
CACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCC436180.13614835194882735No Hit
GCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATT427700.1335014217261531No Hit
GGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAG420340.13120408606119052No Hit
TTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTA408780.1275957708048091No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA399010.12454618256477051No Hit
CCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTT392140.12240179451880683No Hit
CCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAG391070.12206780686099296No Hit
CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC389230.12149347294475232No Hit
ATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCT388190.12116884942687718No Hit
AAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACC359260.11213869714598494No Hit
ATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACG351890.10983824010104282No Hit
CCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTA339700.10603327790594858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)