FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008256
Sequences flagged as poor quality0
Sequence length91
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG728620.5201361254391695Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATAC259160.1850051855134572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG255740.18256376810932068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTGGTGACTCTAGATA197910.14128097030779563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGGCC190700.13613400554644348Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATGACCCGCCGGGCAGCT190390.13591270747764747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC172650.12324874702461176No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC172260.12297033977677164No Hit
GGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT161330.11516779818986746No Hit
GATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT150110.10715823582892832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)