FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16240165
Sequences flagged as poor quality0
Sequence length91
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA810950.4993483748471767Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG752240.4631972643135091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC702110.432329351333561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG646370.3980070399531039Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGGGGCCCGAAGCGTTTA521240.32095733017490896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCGTTTACTTTGAAAAA458020.2820291542604401Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTGTCCCGCGGGGCC433230.2667645310253929Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGCCCGAAGCGTTTAC332020.20444373563938542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATAC279680.1722149990471156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG262940.16190722200174693No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC222220.1368335851267521No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGAAG208640.1284716011198162Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGC203880.12554059641635412No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGGGACGGCCGGGGGCA198800.12241254937988622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATGACCCGCCGGGCAGCT197820.1218091072350558Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGGCC191490.11791136358528377Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGCCC190410.11724634571138902Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT190330.11719708512813755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAACTGAGGCCATGATTAA188850.11628576433798549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT180440.11110724552367542No Hit
TGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAA178130.10968484618228941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGATTAAGAGGGACGGCCG177640.10938312510987418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCCGAAGCGTTTACTT176460.10865653150691512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCGAA166610.1025913221940787Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAA164830.10149527421673364No Hit
GCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCA162440.10002361429209618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)