FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14038477
Sequences flagged as poor quality0
Sequence length91
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC1227720.8745393107813618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG725400.5167227185684031Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGA316310.22531646417200385Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATAC274150.19528471642614795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG262400.18691486263075402Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACG224920.16021680984340395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTGGTGACTCTAGATA198070.14109080351095066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGGCC187150.13331218194110372Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC173480.12357465841914334No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC169040.12041192217645831No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA165810.11811110279270323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT159580.113673299461188No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCG157920.11249083501009405Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAA154660.11016864578686136Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT148110.10550289750091836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)