FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13744127
Sequences flagged as poor quality0
Sequence length91
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC1220580.8880738660229202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG723690.5265449016878264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGA312540.2273989464736465Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATAC267070.19431572481831694Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGGGGCGGGCGCCGG259730.188975261942792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACG225040.1637353903962034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGATGGTAGTCGCCGTGCCT214880.15634314205623973Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTTTGGTGACTCTAGATA196610.1430501915472696Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGGCC186380.13560701236244396Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC171970.12512253415586164No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC169980.12367464299478606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGACGACCCATTCGAA163690.11909814279219043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT158160.11507460604809604No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCG157740.1147690209789243Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAA152790.11116748266368609Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT151080.10992331488205835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)