FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113710
Sequences flagged as poor quality0
Sequence length91
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA825173.903894100893689Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA545882.5825680911761784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAA496762.3501804883356754Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAA348721.6498005875924322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAA297921.4094648745570584Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA290461.3741714804774543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217281.0279555852032682No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAA184070.8708384783153791Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAA173210.8194596231271082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAAAA136180.6442700275818348No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAA114880.5434993447540107Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAA100550.4757038571989535Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAA98530.4661472008932162Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGTTAAAAAAAAAAA94290.4460876846871142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAAAA85320.40365045346807277No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAA78240.3701548462182608Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAAAAAAAAAAAAAAAA72860.3447019695227822Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGTAAAAAAAAAAAA68380.3235070090031272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAA65710.31087519101485067Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAA64790.3065226544795644Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAAA62700.2966348269157074Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAA61500.29095760534794274Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAA61200.2895382999560015Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAAAAA59020.27922468077456225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGAAAAAAAAAAAAA57080.27004650590667595Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTACAAAAAAAAAA53320.25225787832767976Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51370.2430323932800621No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAAAAA47840.22633189983488747No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAAAAA47610.22524376570106588No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAA43280.20475845787738148Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGTGGAAAAAAAAAAAAA43050.2036703237435599Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTCAAAAAAAAAAAA42370.20045323152182654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA42310.20016937044343833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAAA41430.19600607462707753Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAGAAAAAAAAAAAAA40770.1928836027648069Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAAA39220.18555052490644414Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAAA38900.1840365991550402Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAGGAAAAAAAAAAAA38890.1839892889753088Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAAAAAA37600.17788627578996172No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAA36910.174621873388497Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAAA36330.17187788296407738Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAAA35730.16903927218019502Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAA35170.16638990211523813Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33560.1587729631784871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGGGGAAAAAAAAAAAAA32540.15394732484588708Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAAAAAA28240.1336039475613968No Hit
AGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAAAA26660.12612893916383988Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAAAA22910.10838762176457509Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAAAAAA22800.10786720978752998No Hit
AGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAAAA22570.10677907565370842Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGGGGGAAAAAAAAAAAA22430.10611673313746918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA21870.10346736307251232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGTTAAAAAAAAAAAAAA21740.10285233073600447Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGGGAAAAAAAAAAAAA21580.10209536786030249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAA21500.10171688642245152Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGTTAAAAAAAAAAAAA21340.10095992354674954Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)