FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830984
Sequences flagged as poor quality0
Sequence length91
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA325663.918968355588074Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220482.6532400142481682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAA194622.3420426867424644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAA136051.6372156383275729Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA118121.4214473443532005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAA117161.4098947753506685Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81870.9852175252471769No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAA72730.8752274412022374Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAA67740.8151781502411599Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAAAA52650.633586206232611No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAA45620.5489877061411531Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAA40840.49146553964937956Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAA38660.46523158087279665Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGTTAAAAAAAAAAA35920.4322586235114033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAAAA32820.3949534527740607No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAA30950.37245001107121195Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAAAAAAAAAAAAAAAA29310.35271437235855324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGTAAAAAAAAAAAA27630.3324973766041223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAA26320.31673293348608395Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAA25510.3069854533901976Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAAA24670.2968769555129822Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAA24510.2949515273458935Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAA24470.29447017030412137Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGGAAAAAAAAAAAAA23300.2803904768322856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAAAAA23270.28002945905095644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTACAAAAAAAAAA21080.2536751610139305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20570.24753785873133535No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAAAAA19150.23044968374842356No Hit
GTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAAAAA18350.22082254291298029No Hit
CAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAAA17560.21131574133798006Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAGAAAAAAAAAAAAA16760.20168860050253676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAA16690.20084622567943547Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTCAAAAAAAAAAAA16670.2006055471585494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGTGGAAAAAAAAAAAAA16570.19940215455411897Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAAA16030.19290383449019477Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA15780.18989535297911875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAAA15390.18520212182184015Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAGGAAAAAAAAAAAA15350.18472076478006796Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAAAAAAAAA14850.17870380175791592No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAA14810.17822244471614374Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAAA14280.17184446391266256Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAAA14220.17112242835000432Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAA14170.17052073204778911Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13410.161374948254118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGGGGAAAAAAAAAAAAA13100.15764443118038374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAAAAAA11450.13778845320728197No Hit
AGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAAAAAA10790.12984606201804128Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAAAAAA9390.11299856555601552Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGTTAAAAAAAAAAAAAA9090.1093883877427243Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGGGGGAAAAAAAAAAAA8850.1065002454920913Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAAAA8760.10541719214810392Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGGGAAAAAAAAAAAAA8630.1038527817623444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGGGGTTAAAAAAAAAAAAAAAAA8560.1030104069392431Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAAAAAAAAA8550.10289006767880007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA8500.10228837137658488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTAAAAAAAAAAAAAAA8320.10012226468861013Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)