FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118253814
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2816720.23819274023584558Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2089970.17673594866039585Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA1342950.11356504746646058No Hit
CCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAA1326650.1121866564067016No Hit
AACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGC1197290.1012474743520746No Hit
GGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAA1193790.10095150081163556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)