FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139553029
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCGTCG4711766933.76327216803012No Hit
AGATCGGT4520186832.390459973462846Illumina Paired End PCR Primer 2 (100% over 8bp)
GTCATATA4428849631.735961818499835No Hit
TCGGACAC6641820.4759352088301859No Hit
GGCATATA2689530.19272458786974805No Hit
GTCATAAA1445860.10360649355737023No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)