FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006205972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006205972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138160123
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCGTCG4646793633.633392176409686No Hit
AGATCGGT4480833032.43217292156001Illumina Paired End PCR Primer 2 (100% over 8bp)
GTCATATA4383056231.72446654524186No Hit
TCGGACAC6564560.47514144149973003No Hit
GGCATATA3064780.22182811750971007No Hit
GTCATAAA1479540.10708878711695993No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)