FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100120538
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCAT3288524032.84564851219637No Hit
CCGGATCC2952266829.48712480949713ABI Dynabead EcoP Oligo (100% over 8bp)
AACTTAGA2690293926.87054977671015No Hit
TTAACGTG90814729.070538554237494No Hit
CCGGATCA3283990.3280036309832854No Hit
AACTTAAA1676570.167455152907788No Hit
TTAACGTA1576640.15747418376836927No Hit
GGTCGCAA1218040.12165735665543467No Hit
AACTTTGA1021970.10207396208757888No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)