FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99556427
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCAT3276020432.90616687157726No Hit
CCGGATCC2928944329.419941918968224ABI Dynabead EcoP Oligo (100% over 8bp)
AACTTAGA2672224026.84130076303361No Hit
TTAACGTG90212049.061397914571604No Hit
CCGGATCA3266400.3280953423529352No Hit
AACTTAAA1671000.16784451294138952No Hit
TTAACGTA1571080.15780799365168058No Hit
GGTCGCAA1208910.12142962904845911No Hit
AACTTTGA1016910.10214408357583987No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)