FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87796074
Sequences flagged as poor quality0
Sequence length90
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA6380690.7267625657156378Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA2663300.3033506942463054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC2195400.250056739439169No Hit
CTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTA1523000.17347017134274137No Hit
CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA1452750.1654686746015545No Hit
GGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA1275070.14523086761259962No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT1270800.14474451329110682No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA1230770.14018508390249887No Hit
GCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATAT1181530.1345766326635517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGTCTGGTGCCAGCAGCC1160620.13219497719226034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAG1088860.124021490983754Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT1085900.12368434606768407No Hit
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA1072250.12212960684323995No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTG1072070.122109104787533No Hit
GGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT1070460.12192572528926522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCCTTTAACGAGGATC1006730.1146668585659081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGC974660.11101407564078548No Hit
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC962640.10964499392079878No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA953200.10856977499927845No Hit
CATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT943500.10746494199729249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAGTTGCTGCAGTTAAAAA941640.10725308742165396Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG931420.10608902625873681Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGAT923950.10523819094689815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA903530.10291234662725351No Hit
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT900020.10251255654096787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG879390.1001627931563318Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)