FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112385551
Sequences flagged as poor quality0
Sequence length90
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA34714093.0888392405532628Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA11732061.0439117747440683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGAT8573690.7628818761586176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAG6176540.5495848839144811Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAGTTGCTGCAGTTAAAAA6034060.5369070976036768Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT5570770.49568382682930473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC4184590.37234234852841536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAA3866590.34404689620643497Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGAAAGCCGCCGTGGCGCA3697250.32897912294793125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGCTC2936300.2612702410472677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2737290.24356244869947738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC2695000.23979950945829326Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG2665300.23715682098671206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGC2609840.23222202291823085Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGATC2547550.22667949548069574Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGTCTGGTGCCAGCAGCC2453220.2182860677526064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGTTGCTGCAGTTAAA2214100.19700931127703417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG2152680.19154419592604036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAGCTCGTAGTTGGATC2061990.18347465324968687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGTATATTAAAGTTGCTG1990290.17709482956576866Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATAATGGAATAGGACC1934850.17216181108548376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATATTAAAGTTGCTGCAGTT1918840.1707372507343048Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGCT1804020.16052063489905388Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1803840.16050461860528673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG1785000.15882824652432412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTAAAAAGCTCGTAGTTG1748040.15553956753746753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT1731590.15407585624596884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACCAAGGAGTCTAACA1702490.15148655542027817Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1681620.14962955513738593Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC1583080.1408615240939647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGATCT1517270.13500578913387185Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCCTTTAACGAGGATC1498450.13333119664110557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGAT1469950.13079528346130545Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGTCTGGTGCCAGCAGCCG1459260.12984409357035587Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGGGACGGCCGGGGGCA1436720.12783849767306832Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACCTCGGGCCGATCGCAC1422020.12653050034875035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGATCCCG1254470.11162200023382009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCCCCGAATCCGGAGTG1221340.10867411238656471Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAGGGCGGCGGCGGCGGC1220770.10862339412296872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAAAGCTCGTAGTTGGATC1198510.10664271246043008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTA1191060.10597981585728933No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC1155340.10280147133860651Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGCCGCGGTAATTCCAG1137290.10119539299139975Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATCGAACCATCTAGTAGC1130130.1005583004171061Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)