FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113944571
Sequences flagged as poor quality0
Sequence length90
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA35131773.083233338076283Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA11930241.0470213626939717Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGAT8670750.7609620997212758Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAG6243800.5479681870933544Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAGTTGCTGCAGTTAAAAA6089820.5344545989821665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT5653440.4961570305969207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC4253240.3732727204703768Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAA3917180.34377943289636853Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGAAAGCCGCCGTGGCGCA3800430.33353322292116927Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGCTC2975640.2611480278424147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC2918860.2561649031966604Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2802600.24596169658666756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG2676790.23492036316499887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGC2631930.23098336119936774Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGATC2595190.22775898642858552Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGTCTGGTGCCAGCAGCC2483520.21795860726001592Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGTTGCTGCAGTTAAA2242080.19676935726933406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG2175500.1909261653194517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAGCTCGTAGTTGGATC2098350.1841553293486883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGTATATTAAAGTTGCTG2012810.17664817045122758Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATAATGGAATAGGACC1955410.1716106333841917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATATTAAAGTTGCTGCAGTT1938870.17015905040355103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGCT1823720.16005325958004618Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1823660.1600479938618576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG1799810.1579548708819133Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTAAAAAGCTCGTAGTTG1772670.15557301102129736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACCAAGGAGTCTAACA1770040.15534219704069974Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT1754130.15394590410103873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1701360.14931470495421847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC1608780.14118970178930246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAGCTCGTAGTTGGATCT1532770.13451891446412134Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAATCCTTTAACGAGGATC1519130.13332184119592674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGAT1493390.13106284809304342Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGTCTGGTGCCAGCAGCCG1477270.12964812513972254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACCTCGGGCCGATCGCAC1452990.12751726451276033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGGGACGGCCGGGGGCA1449780.12723554858967348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGATCCCG1280540.1123827128192005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCCCCGAATCCGGAGTG1256750.11029485555744467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGAGGGCGGCGGCGGCGGC1252250.10989992669330423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAAAAGCTCGTAGTTGGATC1213690.1065158251374697Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTA1211470.10632099356449375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC1179820.1035433272200393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC1177040.1032993489439703Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATCGAACCATCTAGTAGC1165360.10227428913660135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGCCGCGGTAATTCCAG1148980.10083674807113013Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)