FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006206520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223412425
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGACT6249480127.97284036463057No Hit
TCCTATGA5160960023.100595233232887No Hit
GATGCCTC4879500921.840776760737455No Hit
ATACTGAG4062444818.18361176644495No Hit
ATACGGAG159150437.123615886627613No Hit
ATCCTGAG4148140.1856718577760391No Hit
GATGCCTA3166170.14171861748512868Illumina PCR Primer Index 3 (100% over 8bp)
CGGAGACA2604310.11656961335073464No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)