FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225378041
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCCAACACCGTGC11248910.49911295484194934No Hit
TCTATGGGAGCTCTTC9233560.4096920870831422No Hit
AGCAACTTGCGTTAGT8200230.3638433435491615No Hit
ACAAGACCTTGCGTGC7854780.3485157633435992No Hit
AGAGGGACTCCTAGGC7176840.3184356367708423No Hit
GTTTGAGACGCATGAG7066510.3135403062625786No Hit
AAACCGTACTGCTCAA6954200.3085571233623421No Hit
TTGCGTCACTTTCGCC6945750.30818219775013483No Hit
AGAGTACCTCTGAGAG6882860.3053917750576242No Hit
GTCTACAGAAGCCATT6794640.3014774629263904No Hit
TTCTAGTACCCGCTAG6788170.30119038970615597No Hit
GGGGGGGGGGGGGGGG6469580.28705458487856855No Hit
CGATATTCTACTAGTC6408780.2843568952664737No Hit
TTCCGGACTTAGGCGA6400880.284006373096481No Hit
CTTATTGTGCGATTCA6387810.2834264585696705No Hit
CACTCTGACGGTGCAA6293640.2792481455635689No Hit
CATTACTTGCAAGTTC6293440.2792392715845817No Hit
TGGACTATGTCGACAT6229860.2764182336645654No Hit
CACTCTGACATCATGC5793470.25705565521354407No Hit
GGTGTAACTGAATAAC5586090.2478542264017638No Hit
TACAACCGAGGCAGTA5328370.23641921707891678No Hit
GTGTACTGAACAACAA5249480.23291887606743375No Hit
AACCACTGAGACAAAT4973000.2206514875155916No Hit
ACTCTAACTGCTGTTA4965220.22030628973299135No Hit
GTTGTTGGAAGAGGCT4951710.21970685245240906No Hit
AGGGACCCTAGCCTAT4877930.21643324160404784No Hit
GTTACCCTGACCGTGC4695010.20831710042239654No Hit
TAGCATTCTGTCGGTC4673060.20734318122855633No Hit
TATGGTTCTGGGTAGT4496550.1995114510734433No Hit
GTGCATAACGAAGTTA4416140.19594366782165795No Hit
ATGTGTGTGGTTAGAA4377690.1942376453613775No Hit
GTTGTGATGCTCGATA4146030.18395891550055668No Hit
TCGGGTTCTAGTGAGC4125700.18305687553651245No Hit
AGGGAGTTGCGAACAG4123440.1829565995739576No Hit
TCTATGGGAGTTTGGT3790150.1681685572908143No Hit
ATATTCGCTGGTTGAG3710860.164650468321357No Hit
AAAGCATGATCCTACC3706480.16445612818153832No Hit
GGTCTAAACGTGAGCA3629930.16105961272420502No Hit
GAGTCATTGCAGGGAT3600780.15976623028682727No Hit
CTCTGGACTCGAACAG3597080.15960206167556493No Hit
AGGTGATCTCCTCGTT3590280.15930034639000168No Hit
AAGCCTAACTGCGCCA3581440.15890811651876946No Hit
CGACGACACTGTGCTA3535110.15685245928639516No Hit
ACGGAATCTCCTAGGC3517880.1560879659966518No Hit
TTGTGGTGAACGTAAA3482870.15453457597495046No Hit
AACGAACCTTCTGCAA3448070.1529905036311856No Hit
CAACGCGTGTTCTGAG3410410.15131953338790446No Hit
GCGCAGTTGCCGCTAG3403650.15101959289813863No Hit
CGTGTCTTGACACCGC3381280.15002703834842543No Hit
TCCGTTGACTGCTACA3369620.14950968537347434No Hit
GAATCAGCTAGGGCTA3363800.14925145258494815No Hit
ATTGCTACTCTGTACA3291070.1460244301262695No Hit
CTAGTGCCTCATGCAT3290050.145979172833435No Hit
GATACGACTACTCGAT3274030.14526836711656396No Hit
GCTGGACACAGTGGTG3268940.14504252435134085No Hit
GGAACTACTCCCGAGT3210150.14243401822806684No Hit
TATTCCTGATTGGGAG3194840.14175471513660018No Hit
TCAACAGACCTGGAAG3166200.1404839613456397No Hit
AGTCGAACTTGTTGTG3162290.14031047505644084No Hit
AACCCTCACCCAGCAC3125000.13865592167428592No Hit
GCTTGTCACTGGCATT3071730.13629233737105736No Hit
CAAGATAGAGCTTTGA3050900.13536811245954525No Hit
CCATGATGACCAAGTT3042350.1349887498578444No Hit
CCTGCTATGGAAGGCT3042320.13498741876099632No Hit
GGATTAAGAATTGCCA3037340.1347664566842162No Hit
ATACAGGCTTTTCTTG2995720.13291978165698937No Hit
TGTACCAGACTCCTAC2976150.13205146281309632No Hit
GGTAGCAGATGTGGGA2964860.13155052669927148No Hit
GTGCATAACATCCGTA2960190.1313433192899214No Hit
CCTTACTCTAGGCATG2932540.13011649169494735No Hit
TCGCGGTGACCTAACA2927820.12990706579085048No Hit
TCGCCTCCTGTTCTCC2920980.12960357570948983No Hit
GGTCAGAGAATCCTCC2909570.12909731520827267No Hit
TCCCACATGTTCAGTG2891700.12830442518577043No Hit
TTGTAACCTCGCACTG2885940.12804885459094037No Hit
TGTCGAAACCTGTGCG2856900.12676035284200557No Hit
CCAGTTGCTTGGCAAG2843890.1261831005088912No Hit
ACGCAGAGATTCTGGA2809220.12464479625146799No Hit
CGCACTTACCGCACTG2731780.12120879158764185No Hit
ATGATGGCTGCACCCA2707560.12013415273229747No Hit
TCAATGGACGGAATTA2664360.11821737327107214No Hit
CACCGTTACGCACGCT2607580.11569805063661902No Hit
CGTGTCTTGTCTGTGA2592490.11502850892203824No Hit
AAACGACCTGCGTATT2592130.11501253575986135No Hit
CACCCATACCGACATT2563410.113738232377306No Hit
TCCGACACTAGCGCTC2556090.11341344474637616No Hit
ACTACGCCTGGGTTCC2549890.11313835139777437No Hit
CCACATGCTGGTAGAC2544040.1128787875124001No Hit
GTAATCGTGCCTACGG2529850.11224917870326151No Hit
AGCACATTGCATGCGC2521580.11188223967214267No Hit
AGTTCACGAGCGCACT2505630.11117453984791713No Hit
TTCTCTCTGTGCTACA2475980.10985897246307151No Hit
AGCTTGCCTCTTAGTG2475310.10982924463346452No Hit
TCCGAGCGAAGTCTAC2433410.1079701460356557No Hit
CTAACAGTGACCAGTT2431030.10786454568570858No Hit
ACTGATTCTGCCCTTT2429680.10780464632754527No Hit
TGCATGCCTCGGGTAA2420880.10741419125211049No Hit
CGACCTTACGATGGAC2414810.10714486598985037No Hit
TCGTTCGTGCGATTGT2412860.10705834469472561No Hit
GTTATGCGACCTCCTA2411620.10700332602500526No Hit
CAGTGTAACTTACGAC2408100.10684714399483133No Hit
GAAGCACGATAGCTTG2401030.10653344883763544No Hit
GAACGTTTGTTGCAGT2385550.10584660286402968No Hit
ACTACGCCTTACGATC2377120.10547256464972113No Hit
CTTCAGCACTGCAAGG2368960.10511050630704524No Hit
ACTGCTAGATAGCGAG2349810.10426082281902521No Hit
TCACCCATGGATCCAT2341050.10387214253938785No Hit
ACCCTTTCTGGATGGT2339010.10378162795371887No Hit
CTACAACGATCCTACC2334990.10360326097607708No Hit
TGCCGTTACAACGTGG2324820.10315201914458028No Hit
GGGAGCTCTGCGGTAA2302270.10215147801377865No Hit
TCGACTAGAAGCCATT2297370.10193406552859334No Hit
ATTAGCGTGGCCGCTA2295080.10183245846919044No Hit
CAATCGTGAACAGGCA2290680.10163723093147305No Hit
ATCTGTACTACCGTGC2277630.10105820380256123No Hit
TACTGGAACGCTCTTC2267250.10059764429312791No Hit
GTATTCACTTCGGGCT2264240.10046409090937124No Hit
GGAATGGTGGCTGTTA2260680.1003061340833999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)