FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120777991
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1553970.12866334231374987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1429140.1183278499805482Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA1383750.11456971494086202No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC1332360.11031480064939977No Hit
GAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATAT1316860.10903145424897819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1307990.1082970489217692Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG1301820.10778619425785944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)