FastQCFastQC Report
Sat 25 Jun 2022
EGAF00006206603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184007634
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4525780.24595609984311845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAA3677190.1998389914627129Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA2964000.16108027344126385Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2775040.15081113428152662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2712200.14739605857874352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2555280.13886815152462642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA2261940.12292642162878958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATAT2226800.12101671825202644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2028280.11022803543031263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG1918500.10426197860899618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAA1851300.10060995621518616Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)