FastQCFastQC Report
Fri 24 Jun 2022
EGAF00006206607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171473558
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4244170.24751163091862827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAA3475190.202666232656116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA2779230.1620792169017686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2578520.15037420521710992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2573660.15009077959413428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2429420.1416789870307584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA2127900.12409493480038478Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATAT2087830.12175813136157121No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1918040.11185631314654357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG1791650.10448549740829428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTAAAAAAAAAAAAA1739250.1014296326667462Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)