FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006206675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66097234
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2583210.3908196824091005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1055710.15972075321639026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA812330.12289924265212065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA793160.11999897000228481Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG706280.10685469833730107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)