FastQCFastQC Report
Sun 12 May 2024
EGAF00006206767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58915052
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCGAGAGTGTAGATCT4328190.7346492709537115Illumina Single End Sequencing Primer (100% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCGAGAGTGTAGATC2006960.340653183162768Illumina Single End Sequencing Primer (100% over 33bp)
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG692880.11760661774515621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)