FastQCFastQC Report
Mon 24 Oct 2022
EGAF00006206891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312835445
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA3999120.1278346192516644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3981680.12727713766577825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGG3413280.10910784102485574No Hit
GTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAA3350290.10709432238408918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3307290.10571979783173227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTT3211650.10266259950179239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)