FastQCFastQC Report
Fri 10 May 2024
EGAF00006206895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006206895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332879697
Total Bases33.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15084880.45316311375998397Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12139710.3646876066460731Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCA5614040.1686507182803642Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGC4598200.13813398778718547Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGG4525880.1359614311352849No Hit
GTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAA3896310.11704859248294738No Hit
GCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTG3728940.11202064991064925No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA3703180.1112467967669413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)